Journal: PLoS ONE

Article Title: Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish

doi: 10.1371/journal.pone.0232457

Figure Lengend Snippet: A, Different ECG waveform with distinct P, R, S, and T waves when probes were at a different location relative to the heart (red dots). We recommend aligning the positive probe to the heart (ii), whereby the ECG waveform is similar to that in humans. B, Microsurgery was conducted to open the pericardium sac and thus eliminate noise and enhance amplitude of the waveforms by about 10-fold. C, Change in heart rate with surgery. bpm indicates beats per minute; WT, wild-type.

Article Snippet: ECG signals were then obtained with the ECG recording and analysis System, according to instructions (ZS-200, iWorx Systems, Inc).

Techniques:

Journal: PLoS ONE

Article Title: Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish

doi: 10.1371/journal.pone.0232457

Figure Lengend Snippet: A, Representative electrocardiogram (ECG) for a single fish without anesthesia. Chamber temperature was changed from 22°C to 32°C. B, Heart rate significantly increased with increased chamber temperature, which is independent of anesthesia. Inset shows in-house temperature-controlled chamber. C, Representative ECG for a single fish before and after anesthethia. Temperature was controlled at 28°C. Noise caused by gill movement is reduced after 6 minutes of anesthesia. D, Heart rate to the time after anesthesia. E, Schematic of protocol for conducting ECG with the iWorx system. bpm indicates beats per minute.

Article Snippet: ECG signals were then obtained with the ECG recording and analysis System, according to instructions (ZS-200, iWorx Systems, Inc).

Techniques:

Journal: PLoS ONE

Article Title: Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish

doi: 10.1371/journal.pone.0232457

Figure Lengend Snippet: A, Electrocardiographic (ECG) waveforms with distinguishable P, QRS, and T waves were similar in zebrafish, mice, and humans. B, Heart rate variation (HRV) was measured by quantifying average PP intervals every 30 beats in a representative fish, mouse, and human. HR in the zebrafish was similar to that in a human but was much higher than that in a mouse. HRV in zebrafish was larger than that in both a mouse and a human. C, HRV was measured by quantifying PP i /PP i+1 (ratio of 2 adjacent PP intervals) in a representative fish, mouse, and human. The ratio in a zebrafish was much larger than that in both a mouse and a human.

Article Snippet: ECG signals were then obtained with the ECG recording and analysis System, according to instructions (ZS-200, iWorx Systems, Inc).

Techniques:

Journal: PLoS ONE

Article Title: Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish

doi: 10.1371/journal.pone.0232457

Figure Lengend Snippet: A, Summary of electrocardiographic (ECG) data from 59 wild-type (WT) and 16 Tg(SCN5A-D1275N) fish at 1 year old. Different cut-offs of PP intervals were used to determine an episode of SA. B, Two wild-type (WT) fish and 3 Tg(SCN5A-D1275N) fish that were considered to have SA when 1.2 seconds was used as cut-off, but not if 1.6 seconds was used as cut-off, were further analyzed by reviewing the raw ECG. We considered WT fish 1 and 2 and Tg fish 2 as SA-positive, and others were not because of low frequency of long PP interval. An PP interval more than 1.5 seconds was recommended as a criterion to define SA in zebrafish. C, Representative SA episodes in a WT fish and a Tg(SCN5A-D1275N) fish. epm indicates episodes per minute.

Article Snippet: ECG signals were then obtained with the ECG recording and analysis System, according to instructions (ZS-200, iWorx Systems, Inc).

Techniques:

Journal: PLoS ONE

Article Title: Cerebral cortex and autonomic nervous system responses during emotional memory processing

doi: 10.1371/journal.pone.0229890

Figure Lengend Snippet: The emotional audiovisual recall task required the participants to watch a video footage for 40 seconds (Watching) and recall the contents of the videos with their eyes closed for 180 seconds (Recalling). The participants were presented with pleasant stimuli in session 1 and with unpleasant stimuli in session 2. In the beginning of each session, a quiescent state with the eyes closed was imposed (Control) for 120 seconds in session 1 or 180 seconds in session 2. This sequential watching-and-recalling epoch was repeated 3 times (epochs 1, 2, and 3) with the same kind of stimuli. EEG and ECG were analyzed during the Control and Recall periods. The subjects were instructed to breathe naturally during EEG and ECG recordings.

Article Snippet: EEG and ECG recordings were simultaneously performed using a Nihon-Kohden Inc. system (Tokyo, Japan) with 19 EEG electrodes positioned according to the international 10/20 system (Fp1, Fp2, F3, F4, C3, C4, P3, P4, O1, O2, F7, F8, T3, T4, T5, T6, Fz, Cz, Pz) and with ECG electrodes placed on the right wrist and the left ankle (Lead II).

Techniques: Control

Journal: PLoS ONE

Article Title: Cerebral cortex and autonomic nervous system responses during emotional memory processing

doi: 10.1371/journal.pone.0229890

Figure Lengend Snippet: (A) A series of RR intervals ( RRI n ) was derived from the ECG. (B) After the spline interpolation, resampling was conducted. (C) Spectral analysis using a fast Fourier transform was applied to the resampled time series of the RR interval for about 60 s (59.392 s) at intervals of 2.048 s. (D) The absolute power spectra values in low frequency (LF, 0.04–0.15 Hz) and high frequency (HF, 0.15–0.40 Hz) components were obtained. (E) The power spectra of LF/HF were calculated from the power spectra values of LF and HF in fifty-seven or fifty-eight segments to obtain a time series of the power spectra of LF/HF ( X C ).

Article Snippet: EEG and ECG recordings were simultaneously performed using a Nihon-Kohden Inc. system (Tokyo, Japan) with 19 EEG electrodes positioned according to the international 10/20 system (Fp1, Fp2, F3, F4, C3, C4, P3, P4, O1, O2, F7, F8, T3, T4, T5, T6, Fz, Cz, Pz) and with ECG electrodes placed on the right wrist and the left ankle (Lead II).

Techniques: Derivative Assay

Journal: Sensors (Basel, Switzerland)

Article Title: An Approach towards Motion-Tolerant PPG-Based Algorithm for Real-Time Heart Rate Monitoring of Moving Pigs

doi: 10.3390/s20154251

Figure Lengend Snippet: ( a ) the shimmer Optical Pulse sensing probe (PPG sensor) and data-logger, ( b ) and the ECG recorder, BEAM ® , used to record ECG signals from the pig as a gold standard for heart rate.

Article Snippet: The ECG measurements were performed using a portable ECG recorder: BEAM ® ECG 3-channels ( b) Loop/Event recorder (IEM GmbH; Stolberg, Germany).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: An Approach towards Motion-Tolerant PPG-Based Algorithm for Real-Time Heart Rate Monitoring of Moving Pigs

doi: 10.3390/s20154251

Figure Lengend Snippet: Estimated heart rate from PPG signal vs. heart rate from the gold standard (ECG) measured from the anaesthetised pig′s ear ( a ), the leg ( b ) and the tail( c ).

Article Snippet: The ECG measurements were performed using a portable ECG recorder: BEAM ® ECG 3-channels ( b) Loop/Event recorder (IEM GmbH; Stolberg, Germany).

Techniques: